yanzheng

OVA-Induced Allergic Rhinitis Model

分类：

Model Introduction

The Ovalbumin (OVA)-induced Allergic Rhinitis (AR) model is the most commonly used experimental vehicle in biomedical research. This model utilizes an OVA antigen suspension (usually with aluminum hydroxide gel adjuvant) for systemic sensitization (via intraperitoneal or subcutaneous injection) to induce an immune response, followed by local nasal challenge with OVA solution to simulate an IgE-mediated, non-infectious chronic inflammatory response of the nasal mucosa.

This model is characterized by stable and reliable replication, a high success rate, simple operation, and low cost. It effectively simulates the clinical symptoms (e.g., sneezing, clear rhinorrhea, nasal itching) and pathological features of human allergic rhinitis, serving as a foundation for AR pathogenesis research and drug screening.

Research Applications

  1. Pathogenesis Research: Exploring the role of IgE-mediated immune responses and cytokine networks (e.g., Th2 cytokines) in nasal mucosal inflammation.
  2. Drug Efficacy Evaluation: Used to evaluate the therapeutic effects of clinical drugs—such as antihistamines, glucocorticoids, and leukotriene receptor antagonists—and novel drug candidates.
  3. Pathophysiological Research: Observing nasal mucosal inflammatory cell infiltration, tissue remodeling, and changes in nasal airway resistance.

Key Points of Experimental Design

  • Species: Commonly mice, rats, or guinea pigs.
  • Method: Systemic sensitization combined with local challenge.
  • Key Steps (Mouse IP injection example):
    1. Basic Sensitization Phase: On Days 1, 5, 14, and 21, administer an intraperitoneal injection of OVA suspension (containing 25 μg OVA, 1 mg aluminum hydroxide gel, and 0.5 ml isotonic saline).
    2. Local Challenge Phase: Starting on Day 28 after sensitization, perform bilateral nasal instillation of OVA solution (500 μg OVA in 20 μl isotonic saline) for 10 consecutive days.
  • Alternative Variants:
    • Subcutaneous Injection: Subcutaneous injection of OVA into the footpads or back, potentially adding inactivated Bordetella pertussis to enhance the immune effect.
    • Local Nasal Instillation (Guinea Pigs): Direct local sensitization and challenge via the nasal cavity without systemic injection, avoiding interference from systemic sensitization-induced asthma.

Key Monitoring Indicators

  1. Behavioral Evaluation: Observing and recording typical symptoms such as frequent sneezing, profuse watery rhinorrhea, nasal itching (scratching around the nose), and nasal congestion.
  2. Cytokine Detection: Measuring levels of IL-4, IL-5, IL-6, IL-13, IL-17, and TNF-α in serum or tissue.
  3. Nasal Mucosa Pathological Analysis:
  • Detecting the total number of inflammatory cells and the infiltration area in the nasal septal mucosa.
  • Analyzing the average optical density, integrated optical density, and average blackness of inflammatory cells.
  1. Physiological Indicators (Guinea Pigs): Utilizing the obligate nasal breathing of guinea pigs to reflect changes in nasal airway resistance by monitoring respiratory frequency.
  2. Immunological Indicators: No records available based on current data.

相关产品