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Psoriasis Model

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Model Introduction

The Imiquimod (IMQ)-induced mouse model is currently the most widely used animal model in preclinical psoriasis research. As a drug-induced model, the local application of Imiquimod cream to the animal skin induces skin lesions and inflammatory responses highly similar to human psoriasis. It features low cost, high operability, good reproducibility, a short modeling period, and a high degree of lesion similarity, making it an ideal surrogate for exploring psoriasis pathogenesis and screening new drugs.

Research Applications

  1. Pathogenesis Research: Used to study the roles of immune cells (e.g., dendritic cells) and related inflammatory cytokines (e.g., IL-23/IL-17 axis) in psoriasis-mediated inflammation.
  2. Drug Screening and Evaluation: Assessing the mitigating effects of novel topical or systemic treatments on psoriasis-like lesions.
  3. Immunological Research: Observing the upregulation of pro-inflammatory cytokines and the process of immune cell infiltration under acute inflammation.

Key Points of Experimental Design

  1. Species: Adult mice, typically BALB/c mice, 6–8 weeks of age.
  2. Site: Skin on the back or ears (local depilation required).
  3. Key Steps:
  • Daily topical application of 62.5 mg of 5% Imiquimod cream.
  • Continuous application to the depilated skin of the back or ears.
  1. Cycle: Inflammatory responses generally appear by Day 2–3 and peak on Day 6–7.

Key Monitoring Indicators

  1. Gross Lesion Observation: Observing whether the skin becomes rough, erythematous, wrinkled, or indurated, and whether typical psoriatic manifestations such as plaques, severe scaling, or desquamation are present.
  2. Histopathological Evaluation (H&E Staining):
  • Observation of hyperkeratosis or parakeratosis.
  • Significant thickening of the spinous layer (acanthosis).
  • Reduction or abnormal arrangement of granular layer cells.
  • Proliferation and dilation of small blood vessels in the dermis, and downward protrusion of dermal papillae.
  • Presence of inflammatory cell infiltration.
  1. Immunological Indicators: Detection of pro-inflammatory cytokine expression levels in lesions and the spleen, such as IL-1β, IL-6, TNF-α, and IFN-α.
  2. Cytological Monitoring: Observation of the status of dendritic cells within the lesions and spleen.

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