autoimmune ménière’s disease model #2

Intermittent Hypoxia Model

分类：

Model Introduction

The Chronic Intermittent Hypoxia (CIH) model is a biomedical model that subjects experimental animals to alternating states of hypoxia and reoxygenation by periodically adjusting environmental oxygen concentrations. This model aims to simulate the pathophysiological processes caused by clinical diseases such as Obstructive Sleep Apnea (OSA) and is a core method for studying intermittent hypoxia-induced systemic injury and its mechanisms.

Research Applications

This model is primarily used to simulate pathological changes associated with Obstructive Sleep Apnea (OSA). Specific application directions include:

  1. Metabolic Syndrome Research: Typically simulated using mild CIH (oxygen concentration 10%-15%).
  2. Cardiovascular Injury and Neurodegenerative Disease Research: Typically simulated using severe CIH (oxygen concentration 5%-8%).
  3. Effect Assessment: Short-term (1-2 weeks) exposure is used to study acute effects, while long-term (4-8 weeks) exposure is used to simulate chronic pathological processes.

Experimental Design Key Points

  1. Experimental Animals: SD rats or C57BL/6 mice are commonly used.
  2. Modeling Cycle: Hypoxia treatment is performed at a fixed time daily (e.g., 09:00 to 17:00, for a total of 8 hours) for 5 consecutive weeks.
  3. Modeling Parameters (using a 2-minute cycle):
    • Mild Group: Nitrogen (8 L/min, 40s) → Oxygen (2 L/min, 20s) → Air (6 L/min, 60s), oxygen concentration maintained at 14%-21%.
    • Moderate Group: Nitrogen (10 L/min, 40s) → Oxygen (2.5 L/min, 20s) → Air (6 L/min, 60s), oxygen concentration maintained at 9%-21%.
    • Severe Group: Nitrogen (12 L/min, 40s) → Oxygen (3 L/min, 20s) → Air (6 L/min, 60s), oxygen concentration maintained at 6%-21%.
  4. Environmental Control: The chamber temperature must be maintained at 22-25°C, and humidity at 40-60%.
  5. Operational Precautions:
    • Animals need 1 week of prior environmental adaptation to reduce stress.
    • Diet and water intake for the experimental group animals must be restricted during the modeling period.
    • It is strictly forbidden for the oxygen concentration to drop below 5% to prevent acute death of the animals.

Key Monitoring Indicators

  1. General Signs: Weekly recording of animal body weight.
  2. Biochemical, Pathological, or Behavioral Indicators: No relevant records based on current data.

相关产品