liver fibrosis model

Inflammatory Bowel Disease Model

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Model Introduction

The inflammatory bowel disease (IBD) model is an experimental system used to simulate the pathological features of human ulcerative colitis (UC) and Crohn’s disease (CD). The model system is categorized into in vitro and animal models: in vitro models reconstruct “mini-intestines” by building 3D multilayered structures (containing epithelial, endothelial, and stromal layers) to simulate barrier disruption and matrix remodeling; animal models demonstrate the occurrence and development of intestinal inflammation in vivo through chemical induction, genetic engineering, antibiotic intervention, cell transplantation, or the use of spontaneous genetic susceptibility strains.

Research Applications

  1. Pathogenesis Investigation: Studying the impact of immune cell infiltration, intestinal epithelial barrier damage, extracellular matrix changes (e.g., the roles of MMP-3, MMP-9), and gut microbiota dysbiosis on IBD.
  2. Drug Screening and Evaluation: Utilizing models for efficacy testing of candidate drugs, evaluating their effects on inflammatory factor release, tissue repair, and clinical symptom improvement.
  3. Genetic and Immunological Research: Studying the roles of specific genes (e.g., NOD2, IL-10) or immune cells in the disease through gene knockout or cell transplantation.
  4. Integrative Medicine Research: Simulating Traditional Chinese Medicine (TCM) syndromes (e.g., TCM patterns of Damp-Heat or Spleen Deficiency) to evaluate the therapeutic value of TCM for IBD.

Key Points of Experimental Design

  1. Chemical Induction Models:
    • DSS Induction: 3%-5% DSS solution replaces drinking water for 7 consecutive days.
    • TNBS Induction: TNBS mixed with ethanol is administered into the colon via enema.
    • Acetic Acid Induction: 4% acetic acid solution enema for 5 minutes.
  2. Antibiotic-Induced Models: Administration of varying doses of clindamycin, ampicillin, or streptomycin to disrupt gut microbiota balance.
  3. Genetic Engineering Models: Knocking out or overexpressing IBD-related genes (e.g., NOD2, IL-10) using CRISPR/Cas9 or other technologies.
  4. Cell Transplantation Models: Isolating donor immune cells or intestinal stem cells and transplanting them into recipient animals.
  5. Spontaneous Models: Selecting strains with specific genetic backgrounds (e.g., IL-10 knockout mice).
  6. TCM Syndrome/Pattern Models: Combining chemical induction with TCM etiologies (e.g., Damp-Heat, Spleen Deficiency) for composite modeling.

Key Monitoring Indicators

  1. Clinical Signs: Body weight changes, diarrhea severity, hematochezia, food intake, fecal weight.
  2. Histopathological Evaluation: Colon tissue damage, inflammation score, and immunohistochemical analysis.
  3. Molecular Biological Indicators: Detection of inflammatory cytokines (e.g., TNF-α, IL-6), tight junction gene expression analysis, and matrix metalloproteinase (MMP-3, MMP-9) levels.
  4. Comprehensive Evaluation: Disease Activity Index (DAI), hematological assessment, gut microbiota analysis, and TCM syndrome scores.

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