Cell & Gene Therapy / Viral Vector

The Cre/LoxP system is a site-specific DNA recombination technology that enables spatial and temporal control over gene expression, allowing researchers to perform targeted knockout, inversion, or activation in specific cell types or developmental stages. Derived from bacteriophage P1, the Cre recombinase recognizes directional 34-base-pair LoxP recognition sites. When Cre binds to two flanking LoxP sites […]

A missing target band on a Western blot typically stems from low biological expression, sample degradation during extraction, or technical failures during transfer and immunodetection. Key causes should be investigated in the following order: To isolate the root cause, assess membrane transfer efficiency using reversible total protein staining (such as Ponceau S), verify antibody specificity