In vitro biology provides professional support for drug discovery and development. Targeting has an advanced screening platform and offers multi-level and comprehensive services for partners. The business scope covers target validation, hit compound discovery, transition from hit compounds to lead compounds, optimization of lead compounds, and selection of clinical candidate compounds. With rich experience and advanced technical platforms, we can assist clients in addressing a variety of major disease areas, including oncology, immuno-oncology, metabolic diseases, immune and inflammatory diseases, and neurological diseases.
In Vitro Biochemical Analysis
Technology Platform
- Fluorescence analysis methods: Lance, Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP, etc.
- Luminescence analysis methods: Kinase-Glo; ADP-Glo, etc.
- Absorbance analysis methods: UV, VI
- Label-free screening methods: SPR (Biacore)/ITC
Validated Enzymatic Targets
| Abl | COX-1/COX-2 | HER2 | PI3K alpha |
| AKT1 | C-Raf | IKK beta | PI3K beta |
| AKT2 | DPPIV | JAK1 | PI3K delta |
| AKT3 | EGFR | JAK2 | PI3K gamma |
| ALK | EGFR (L858R,T790M) | JAK3 | PKA |
| Aurora A | Factor Xa | LCK | PKC delta |
| Aurora B | FGFR1 | Lipoxygenase | PKC gamma |
| B-Raf | Flt1/VEGFR1 | Lyn | Plk1 |
| BTK | Flt3 | MAPK14 | 20S proteasome |
| Cathepsin K/L/B/S | Flt4 | MARK1 | Renin |
| CDK/P25 | α-Glucosidase | MEK1 | SAHase |
| CHK1 | β-Glucosidase | MEK2 | SGK1 |
| CHK2 | GSK3 beta | mTOR | SRC |
| c-Kit | H+/K+ ATPase | PDE5 | Syk |
| c-Met | HDAC1 & HDACs | PDGFR beta | VEGFR2 |
Cell-based Analysis
Technology Platform
- Cell cytotoxicity detection
- Cell apoptosis detection analysis
- Cell migration analysis
- Cell invasion analysis
- HTRF cell-based detection analysis
- Immunostaining analysis
- Immunofluorescence analysis (96/384-well plate)
- Reporter gene analysis (green fluorescent protein/fluorescein)
- Radioligand receptor binding assay
- Cell uptake analysis
- Gene knockout analysis
- RNA interference
- MicroRNA overexpression analysis
- MicroRNA knockout analysis
- Application analysis of adenovirus/retrovirus/lentivirus vectors
Western Blot Analysis
Expression level of Phospho-Akt and Akt in mouse tumor
HT1080 Cell and MDA-MB-231 Cell Migration Assay
Radioisotope Analysis
Technology Platform (3H, 33P, 32P, 35S, 125I, 14C)
- Radioligand receptor binding test
- Radioligand binding test
- Radioactive uptake test
- Cell growth test
- Radioimmunoassay analysis
- Immuno-radiometric analysis
Receptor Binding Assay – 3H
3H-5HT uptake assay – 3H
Radioactive Iodine Uptake Test – 125I
